EISSN 2149-6498

ENT Updates INDEXED IN WEB OF SCIENCE

ENT Updates

14 42 2024

HIGHLY CITED ARTICLES

The Score for Allergic Rhinitis study in Turkey, 2020

by Cingi C, et al.

The Effect of Selfies on Cosmetic Surgery

by Alkarzae M, et al.

Prognostic Significance of Inflammatory Markers in Patients with Oral Cavity Cancers

by Erdis and Yucel.

A great mimicker of mastoiditis: Multisystem Langerhans' cell histiocytosis

by Kin FS, et al.

Does Nasal Septum Deviation with Different Locations and Different Angular Features Affect Maxillary Sinus Volumes?

by Avcı and Tiryaki.

Total Nasal Airway Resistance with Different Head Positions in Allergic Rhinitis Patients: A Four-Phase Rhinomanometric Study

by Birdane and Kucukcan.

The Outcomes in Patients Treated with Intratympanic and Systemic Steroids for Sudden Hearing Loss and Prognostic Factors

by Cecen AB, et al.

The Effect of Ear Drainage and Middle Ear Inflammation due to Chronic Otitis Media on Infrared Tympanic Temperature Measurement

by Cengiz C, et al.

The Turkish Short Version of the CSpeech, Spatial, and Qualities of Hearing Scale (SSQ) for Clinical Use: Determining Reliability and Validity for People with and without Hearing Loss on the Basis of SSQ12-A, SSQ12-B, SSQ12-C

Cildir B, et al.

Relationship Between Parathormone and Inflammatory Markers on Complete Blood Count in Parathyroid Adenoma Patients

by Cirakoglu MF, et al.

Clinical Research

Metformin And Dichloroacetate Combination Exert A Synergistic Effect On Cell Viability Of Oral Squamous Cell Carcinoma

Seniz INANC ¹ , Didem KELES ^{2 3} , Gorkem ESKIIZMIR ⁴ , Yasemin BASBINAR ⁵ , Gulgun OKTAY ²

Author Affiliation(s)

1.

Dokuz Eylul University, 1Institute of Health Sciences

2.

School of Medicine, Department of Medical Biochemistry

3.

Izmir Univ. of Economics, Vocational School of Health Services, Dept. of Medical Laboratory Techniques, 35330 Balcova, Izmir-TURKEY

4.

Manisa Celal Bayar University, Faculty of Medicine, Department of Otolaryngology-Head and Neck Surgery, Manisa - TURKEY

5.

Institute of Oncology, Department of Translational Oncology, 35340 Inciralti, Izmir

ENT Updates 2019; 9: 68-73

DOI: 10.32448/entupdates.569464

Keywords : Metformin, Dichloroacetate, drug combinations, oral cavity, cancer

Read: 1013 Downloads: 717 Published: 28 January 2021

Volume 9, Issue 2, August 2019

Previous Article

Objective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line.

Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x104 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability. In addition, drug combination index was analyzed with CompuSyn software according to Chou-Talalay method.

Results: Half-maximal inhibitory concentrations (IC50) of Metformin and DCA were found to be 3mM and 23mM, respectively, for 72 hours. CI values (0.76-0.80) in all combination groups below 1 indicated that Metformin/DCA combination had a moderate synergistic effect on cell viability in UPCI-SCC-131 cells.

Discussion: Metformin/DCA combination synergistically decreased the cell viability of UPCI-SCC-131 cells. Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the “drug repositioning” of the treatment of oral cavity cancer.

Files